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For each query gene, a list of significantly interacting genes was compiled that included all drug conditions and both positively and negatively interacting genes. 2006; Hillenmeyer et al. Software, Interestingly, the converse was observed in the case of HU, in that deletion of RTT107 mildly suppressed the sensitivity of mrc1∆ mutants. Whilst partial knockdown may be desirable in certain situations, a technology with improved targeting efficiency and fewer off-target effects was needed. Our study reveals the great potential of P. patens in forward genetics that was envisioned by the pioneers of this system [1, 2], and similar to Arabidopsis, it guarantees to provide novel insights into many plant biology problems when combined with more sophisticated genetic screening strategies [52]. We anticipate that the combination of haploid genetics, simple development, and reduced genetic complexity will continue to strengthen the role of P. patens as a model land plant to study many aspects of plant growth and development [53, 54]. 1291, Conditional genetic screen in Physcomitrella patens reveals a novel microtubule depolymerizing-end-tracking protein, Xinxin Ding, Worcester Polytechnic Institute S2B), the corresponding overlap with genes that were upregulated in jmj14 root tips was nearly 4-fold over random expectation (Fig. Genes that showed condition-specific interactions with query genes that were statistically significant after multiple test correction (, GO terms that were significantly enriched in the list of genetically interacting genes under all conditions using DAVID. All DNA fragments were amplified from Col-0 genomic DNA or cDNA using suitable oligonucleotides (see Table S6). ‘a’ represents the transcript before splicing of intron 10, ‘b’ the corresponding mature transcripts. Two recent studies used genetic interaction mapping to gain new insights into the DNA damage response (Bandyopadhyay et al. Due to their small genomes and limited number of encoded proteins, viruses exploit host proteins for entry, replication, and transmission. 2007). https://doi.org/10.1371/journal.pgen.1007221.s001, https://doi.org/10.1371/journal.pgen.1007221.s002. This page was last edited on 19 October 2020, at 09:31. (H) Confocal microscopy images of a root meristem area expressing JMJ14-CITRINE fusion protein (green) under control of the JMJ14 promoter in Col-0, following fluorescence recovery in the outlined trapezoid area after bleaching over time. Under these conditions caulonemal cells grow for several days and can be observed with high numerical aperture (NA) optics. We would like to thank the Lausanne Genomic Technologies Facility for whole-genome sequencing and RNAseq services. During the mutant selection process, the plates were expanded to accommodate the four different conditions tested and to measure each condition with three technical replicates (see Figure 1 for workflow). Moreover, there is some evidence suggesting that Hrq1 and Slx4 are both involved in interstrand crosslink repair and suppression of telomere addition (Zhang et al. Scale bar indicates substitutions per site. We thus conclude that JMJ14 inhibits root meristem activity upon suboptimal root growth in tissue culture. The resulting DNA fragments were separated by gel electrophoresis, gel-extracted and Sanger sequenced directly. In order to extract approximately the same amount of genomic DNA from each of the 24 F1 segregants of TS mutant clog1, approximately 0.2 g of one-week old moss was weighed for each segregant. Low levels of CLoG1 protein are consistent with a low level of transcript expression deduced from the transcriptome atlas of P. patens [39].

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conditional genetic screen

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